Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics.
At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial
pathogens in various specimens.
MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.
Taylorella equigenitalis causes contagious equine metritis (CEM), which is an inflammatory disease of the proximal and distal reproductive tract of the mare and usually results in temporary infertility. OIE has listed CEM as a notifiable disease. Clinical signs include endometritis, cervicitis and vaginitis of variable severity and a slight to copious mucopurulent vaginal discharge. Direct venereal contact during natural mating presents the highest risk for the transmission of T. equigenitalis from a contaminated stallion or an infected mare. Direct venereal transmission can also take place by artificial insemination using infective raw, chilled and possibly frozen semen. Indirectly, infection may be acquired through fomite transmission, manual contamination, inadequate observance of appropriate biosecurity measures at the time of breeding and at semen collection centres. Stallions can become asymptomatic carriers of T. equigenitalis. The principal sites of colonisation by the bacterium are the urogenital membranes (urethral fossa, urethral sinus, terminal urethra and penile sheath). The sites of persistence of T. equigenitalis in the majority of carrier mares are the clitoral sinuses and fossa and infrequently the uterus. Foals born of carrier mares may also become carriers. The organism can infect equid species other than horses, e.g. donkeys.
Taylorella asinigenitalis is a microaerophilic, non-motile, coccobacillus, Gram-negative bacterium: It is closely related to Taylorella equigenitalis and mainly found in donkeys. It does not cause apparent disease in mares.
Pathogens Tested
This assay can detect and differentiate between Taylorella equigenitalis and Taylorella asinigenitalis.
Method
Real-Time PCR
Sample Type
Accredited :
Culture.
Alternatives :
Swab/Secretions (Genital).
Transport Condition
Samples should be transported at 4°C.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Theileriosis is a diseases caused by Theileria annulata (T.annulata), an intracellular protozoa. It is usually transmitted by ticks and through infected water. Theileriosis have a high impact on the health, welfare, and productivity of livestock species with the highest prevalence in tropical subtropical climate.
A secretion of infective sporozoites during tick feeding into feeding sites of mammalian species and clinical signs include fever, slight nasal and ocular discharge, anorexia, salivation, enlargement of superficial lymph nodes. Infection can be controlled by drugs or vaccines, although these are not fully efficient.
Diagnosis of the disease based on microscopic examination of Giemsa stained blood smears and lymph node biopsy is disadvantageous in case of low parasitemia and carrier state animals. So, the specific and sensitive molecular method is used to detect the parasite infection is PCR.
Method
Real Time PCR
Sample Type
Recommended specimen types: Tissue or EDTA Blood (>=3mL).
Transport Condition
Sample should be transported at 4oC. (Refer to MBG-C0014)
Turn Around Time (TAT)
The Turnaround (TAT) for routine samples is within 3 working days.
Samples delivered before 11:00 AM will begin processing immediately resulting in shorter TAT.
Urgent Samples must be delivered before 11:00 AM and will be charged double.
Trypanosoma evansi and T. brucei are flagellated protozoan parasites that live in the blood, lymph and various tissues of their vertebrate hosts. Trypanosoma evansi causes a trypanosomosis known as "surra". It has a wide host range. In some countries incidence of surra increases significantly during the rainy season when biting fly populations have greatly increased. Surra affects mainly camels and horses but buffaloes and cattle are also affected. Other species that develop severe disease include donkeys, mules, deer, llamas, dogs, cats, cattle and buffalo. Sheep, goats, pigs and elephants may occasionally develop mild or chronic disease.T. evansi has a wide distribution in Asia, North Africa (extending into tsetse areas with T. brucei infections) and Central and South America.
T. evansi is transmitted from animal to animal by mechanical vectors such as hematophagous flies, including Tabanus spp. and Musca spp., as well as Lyperosia, Stomoxys and Atylotus genera. Tabanids (horse flies) are the most significant vectors. Infection occurs through blood from infected animals and occasionally through meat and milk. T. evansi frequently localizes extravascularly in tissues including the central nervous system. The disease is characterized by recurrent episodes of fever and parasitaemia. Abortions have been reported in buffaloes and camels.
Pathogens Tested
This assay is used for the detection of both Trypanosoma evansi and Trypanosoma brucei, however, it cannot differentiate between the two subtypes.
Method
Real-Time PCR
Sample Type
Accredited :
EDTA blood, Culture.
Alternatives :
Tissue.
Transport Condition
Samples should be transported at 4°C.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics.
At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial
pathogens in various specimens.
MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.
Taylorella equigenitalis causes contagious equine metritis (CEM), which is an inflammatory disease of the proximal and distal reproductive tract of the mare and usually results in temporary infertility. OIE has listed CEM as a notifiable disease. Clinical signs include endometritis, cervicitis and vaginitis of variable severity and a slight to copious mucopurulent vaginal discharge. Direct venereal contact during natural mating presents the highest risk for the transmission of T. equigenitalis from a contaminated stallion or an infected mare. Direct venereal transmission can also take place by artificial insemination using infective raw, chilled and possibly frozen semen. Indirectly, infection may be acquired through fomite transmission, manual contamination, inadequate observance of appropriate biosecurity measures at the time of breeding and at semen collection centres. Stallions can become asymptomatic carriers of T. equigenitalis. The principal sites of colonisation by the bacterium are the urogenital membranes (urethral fossa, urethral sinus, terminal urethra and penile sheath). The sites of persistence of T. equigenitalis in the majority of carrier mares are the clitoral sinuses and fossa and infrequently the uterus. Foals born of carrier mares may also become carriers. The organism can infect equid species other than horses, e.g. donkeys.
Taylorella asinigenitalis is a microaerophilic, non-motile, coccobacillus, Gram-negative bacterium: It is closely related to Taylorella equigenitalis and mainly found in donkeys. It does not cause apparent disease in mares.
Pathogens Tested
This assay can detect and differentiate between Taylorella equigenitalis and Taylorella asinigenitalis.
Method
Real-Time PCR
Sample Type
Accredited :
Culture.
Alternatives :
Swab/Secretions (Genital).
Transport Condition
Samples should be transported at 4°C.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Theileriosis is a diseases caused by Theileria annulata (T.annulata), an intracellular protozoa. It is usually transmitted by ticks and through infected water. Theileriosis have a high impact on the health, welfare, and productivity of livestock species with the highest prevalence in tropical subtropical climate.
A secretion of infective sporozoites during tick feeding into feeding sites of mammalian species and clinical signs include fever, slight nasal and ocular discharge, anorexia, salivation, enlargement of superficial lymph nodes. Infection can be controlled by drugs or vaccines, although these are not fully efficient.
Diagnosis of the disease based on microscopic examination of Giemsa stained blood smears and lymph node biopsy is disadvantageous in case of low parasitemia and carrier state animals. So, the specific and sensitive molecular method is used to detect the parasite infection is PCR.
Method
Real Time PCR
Sample Type
Recommended specimen types: Tissue or EDTA Blood (>=3mL).
Transport Condition
Sample should be transported at 4oC. (Refer to MBG-C0014)
Turn Around Time (TAT)
The Turnaround (TAT) for routine samples is within 3 working days.
Samples delivered before 11:00 AM will begin processing immediately resulting in shorter TAT.
Urgent Samples must be delivered before 11:00 AM and will be charged double.
Trypanosoma evansi and T. brucei are flagellated protozoan parasites that live in the blood, lymph and various tissues of their vertebrate hosts. Trypanosoma evansi causes a trypanosomosis known as "surra". It has a wide host range. In some countries incidence of surra increases significantly during the rainy season when biting fly populations have greatly increased. Surra affects mainly camels and horses but buffaloes and cattle are also affected. Other species that develop severe disease include donkeys, mules, deer, llamas, dogs, cats, cattle and buffalo. Sheep, goats, pigs and elephants may occasionally develop mild or chronic disease.T. evansi has a wide distribution in Asia, North Africa (extending into tsetse areas with T. brucei infections) and Central and South America.
T. evansi is transmitted from animal to animal by mechanical vectors such as hematophagous flies, including Tabanus spp. and Musca spp., as well as Lyperosia, Stomoxys and Atylotus genera. Tabanids (horse flies) are the most significant vectors. Infection occurs through blood from infected animals and occasionally through meat and milk. T. evansi frequently localizes extravascularly in tissues including the central nervous system. The disease is characterized by recurrent episodes of fever and parasitaemia. Abortions have been reported in buffaloes and camels.
Pathogens Tested
This assay is used for the detection of both Trypanosoma evansi and Trypanosoma brucei, however, it cannot differentiate between the two subtypes.
Method
Real-Time PCR
Sample Type
Accredited :
EDTA blood, Culture.
Alternatives :
Tissue.
Transport Condition
Samples should be transported at 4°C.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
